Feulgen-DNA content of individual bull spermatozoa
نویسندگان
چکیده
منابع مشابه
A nuclear DNA polymerase in bull spermatozoa.
A DNA polymerase was isolated from bull spermatozoa by differential centrifugation, ultrafiltration and gel filtration. Its apparent molecular weight and synthetic template utilization resemble that of DNA polymerase gamma. Chemical and enzymic fractionation of bull spermatozoa indicate that the enzyme is most probably located in the nucleus.
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Individual bull spermatozoa were tracked over a distance of several millimetres, measuring as often as possible the time required to swim the length of the micrometer (310 \g=m\). In oestrous mucus from the cow's uterine cervix, the rates of some spermatozoa were found to fluctuate widely, while others remained constant for long distances. Homogeneity of the medium was tested by comparing mean ...
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The electron microscope has been used to determine the characteristic dimensions and the distribution of the dry mass in bull spermatozoa. The most important result is that all characteristic data are occurring in logarithmic distributions. Furthermore, no correlation between such parameters as head weight and tail weight or head length and tail length was found. The occurrence in logarithmic d...
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It has been known since the nineteenth century that the microscopical structure of living cells may be modified by fixation and by post-mortem or sub-mortem changes (Hardy, 1899), but inadequate attention seems to have been paid to factors which may injure spermatozoa before fixation and microscopical examination. One such factor is the effect of sudden cooling. If semen is exposed to a sudden ...
متن کاملDecrease in Nuclear Feulgen-positive Material (dna) upon Aging in in Vitro Storage of Bovine Spermatozoa
The Feulgen-DNA content of sperm cells from 5 bulls was studied by means of microspectrophotometry after storage at 5 degrees C for 2, 3, 5, and 10 days in a yolk-citrate diluent permitting slow aerobic metabolism. A subsample of sperm cells from each bull was subjected to the Feulgen technique on each of the storage days selected. The cells sampled on each of these days received a standard 12 ...
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ژورنال
عنوان ژورنال: Experimental Cell Research
سال: 1966
ISSN: 0014-4827
DOI: 10.1016/0014-4827(66)90465-4